Request Specialist Information: If you are not sure which HPLC column is most effective suited to your software, consult with with an expert in the sector. Experienced chromatographers can give steering and suggest exceptional column variety based mostly on their information and experience.
20 mL membrane volume, which permits bioprocess customers less difficult scale-up and is an ideal match to the creation of diagnostic products and solutions.
Ion Trade columns is often possibly cationic or anionic in nature. Anion exchange columns keep and independent anions or negatively billed ions. Cation-Trade columns retain and separate positively billed cations.
A pump provides the cellular phase by way of a column full of a stationary phase. An autosampler injects the sample onto the column. The stationary phase separates the sample compounds or analytes. A detector actions the analytes right after separation and elution within the column.
Sartobind STIC® PA can work at substantial conductivity and can remove DNA at up to one.five M NaCl. DNA removal is achievable even inside the existence of phosphate buffers, which provides some one of a kind processing chances.
This band broadening boosts the time demanded for comprehensive elution of a certain compound and is generally undesirable. It must be minimized so that overly broad elution bands will not overlap with each other. We will see how this is measured quantitatively when we focus on peak resolution momentarily.
In this article, the USP L quantity could be selectively chosen and also the suited columns of the present manufacturers is usually compared with each other .
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Get insight into finest tactics for optimization and sizing of AEX membrane adsorbers for purification procedures.
This issue is solved by bonding polar purposeful groups including amino- or cyano-moiety towards the silanol teams of your column.
Tandem liquid chromatography procedures make use of a next pump and smart column switching To optimize utilization on the detector by minimizing downtime associated with column reconditioning.
Ion exchange columns are used to independent compounds based mostly on their charge. The stationary phase check here is often a charged content, like a cation exchange resin or an anion Trade resin. The cellular phase is really a buffer solution which contains ions of the alternative charge for the stationary phase.
The most widely used type of column in HPLC could be the reverse-phase column. These columns use a non-polar get more info stationary phase in addition to a polar cellular phase. This results in the polar molecules to elute a lot quicker from the column, even though non-polar molecules elute slower.
We also give personalized-packed columns to provide you with the optimum adaptability with your HPLC applications. With our custom made packing program, our several particles, chemistry choices, and column components are available in different mixtures.